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. 2008 Jul;10(7):687–696. doi: 10.1593/neo.08314

Figure 1.

Figure 1

Design of the real-time PCR assays that were used for the investigation of HST expression in breast carcinomas. (a) Specific primers and probes were designed for the detection of HST (165-bp product retaining intron 8) and HER2 (150-bp product lacking intron 8, probably corresponding to HER2 receptor) mRNA targets. The sense HST primer includes an S corresponding to the silent G/C mismatch in exon 7 (AF_177761 and NM_004448), which in two of three sequenced cases proved to be a G. Theoretically, a 424-bp product corresponding to HST transcripts would coamplify with the wild type HER2 target; this, however, could not be observed in the FFPE samples. Sequences are shown in 5′-3′ sense direction. (b) Representative RT-PCR results for matched NCBT/tumor pairs are shown. Specific HST products are detected in the presence of HER2.