Figure 5.

Ref-1 depletion by siRNA prevents NF-κB nuclear translocation in LPS-stimulated macrophages. (a) Cells transfected with Ref-1 siRNA were stimulated for 24 hr as indicated in Fig. 3. The cells were fixed, reacted with NF-κB p65 antibody (green) and PI (red) for analysis by confocal microscopy. (b) Nuclear (N) and cytoplasmic (C) proteins were isolated and used for Western blot analysis.