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. 2008 May;124(1):129–140. doi: 10.1111/j.1365-2567.2007.02749.x

Figure 1.

Figure 1

Murine B-cell populations can be expanded by stimulation with CD40L. Spleen cell populations were phenotyped for B220 expression and cultured in duplicate wells for 12 days on CD40L-transfected L cells in the presence of rIL-4. Duplicate wells were harvested on days 4, 8, and 12 and the number of viable (trypan blue excluding), B220+ B cells determined. Data represent the total number of B220+ B cells recovered at various time points in 8 independent cultures. A representative B220 phenotype of cells after 7 days of culture is presented in the insert.