Figure 1.

The mRNA and protein expression level of frizzled family members in colon cancer cell lines. (A) The extracted total RNA was reverse-transcribed into single-stranded cDNA. Real-time PCR was performed using first-strand cDNA with TaqMan. GAPDH was used as an endogenous control. Data are presented as mean values and SD for three independent experiments compared with the level of FZD1 that is presented as 1. (B) Western blot analysis of protein expression of FZD7 in six colon cancer cells. 293T cells was transfected with FZD7 as a positive control.