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. Author manuscript; available in PMC: 2008 Jun 20.
Published in final edited form as: Neuron. 2007 Dec 20;56(6):1076–1089. doi: 10.1016/j.neuron.2007.11.015

Figure 5. Acute Blockade of Ih Prolongs Duration of the Long-Lasting Dendritic Depolarization in Response to PP but Not SC Stimulation.

Figure 5

(A) Schematic of the recording configuration. Whole-cell current-clamp voltage recordings were obtained from the apical trunk of a CA1 pyramidal neuron. Extracellular electrodes were positioned to stimulate either PP inputs in SLM or SC inputs in SR. (Inset) Brightfield Alexa 594 fluorescent image of a representative recording. Soma of patch-clamped dendrite is visible in the CA1 pyramidal cell layer. Scale bar, 20 μm.

(B1) Voltage responses to low, intermediate, or strong PP stimulation from a whole-cell dendritic recording obtained ~200 μm from the center of the soma, in the absence (black traces) or presence (red traces) of ZD7288 (10 μM).

(C1) Voltage responses in absence or presence of ZD7288 to low, intermediate, or strong PP stimulation from a second dendritic recording ~85 μm from soma.

(D1) Dendritic voltage responses to SC stimulation from same recording shown in (C) in the absence and presence of ZD7288.

(B2–D2) Plots of half-width duration of voltage responses as function of stimulus intensity in the absence (black circles) or presence (red circles) of ZD7288 for experiments shown in (B1)–(C1).