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. Author manuscript; available in PMC: 2008 Jun 20.
Published in final edited form as: Mol Cell Proteomics. 2007 Oct 15;6(12):2212–2229. doi: 10.1074/mcp.M700354-MCP200

Fig. 2. Calibration curve for quantifying mouse leptin in MARS hu7 and IgY-12 depleted plasma.

Fig. 2

The area ratio of light peptide to heavy peptide was determined from the extracted ion chromatograms of the 467.2/643.8 and 468.9/646.3 transitions, respectively, and plotted versus protein concentration. Three biological replicates for each concentration point were generated in MARS hu7 (closed triangle) and IgY-12 (closed square) depleted plasma and analyzed in triplicate by MRM (n = 9). Error bars indicate S.D. of the measurements. Raw data are shown in Fig. 3.