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. Author manuscript; available in PMC: 2009 May 1.
Published in final edited form as: Plasmid. 2008 Mar 4;59(3):193–201. doi: 10.1016/j.plasmid.2008.01.004

Fig. 1.

Fig. 1

New cloning vectors constructed from pSC101 repA elevated copy number mutants. Shown are unique restriction enzyme recognition sites; lacZα, encoding the α peptide from β-galactosidase; bla (AmpR); kan (KanR); and cam, (CamR). The sizes of each plasmid in kb are also shown. The expanded region below repA shows the relevant sequences of the gene and product from wild type pSC101 along with the repA (BglII) mutation. Also shown are the two high copy number mutations. The restriction sites used to construct the vectors are underlined and the sequences encoding amino acid position 93 are shown in bold. The copy number of each replicon is also shown. The position of the EagI site used in constructing the vectors is shown, however, this site is not unique.