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. Author manuscript; available in PMC: 2009 May 1.
Published in final edited form as: Plasmid. 2008 Mar 4;59(3):193–201. doi: 10.1016/j.plasmid.2008.01.004

Table 1.

The plasmids and strains constructed and used in this work

Plasmid or E. coli Strain Relevant Genotype Source or Reference
Plasmids
pWSK30 repA+ (Wang and Kushner, 1991)
pCL21 repA+ (Lerner and Inouye, 1990)
pCL21-BglII repA-BglII (silent mutation) This work
pBR322 bla Lab collection
pBADffh bla, ffh Lab collection
pJPA12 repAE93K, bla This work
pJPA13 repAE93R, bla This work
pJPC12 repAE93K, cam This work
pJPC13 repAE93R, cam This work
pJPK12 repAE93K, kan This work
pJPK13 repAE93R, kan This work
pWSKffs repA+ffs+ This work
pJPA11ffs repA-62, ffs+ bla This work
pJPA12ffs repAE93K, ffs+ bla This work
pJPA13ffs repAE93R, ffs+ bla This work
Strains
NEB5α fhuA2Δ(argF-lacZ)U169 phoA
glnV44 Φ80 Δ(lacZ)M15 gyrA96
recA1 relA1 endA1 thi-1 hsdR17
(general cloning host)		 New England Biolabs
SKP1101 ffhTS, cam (Park et al., 2002)
SKP1102 ffh+, cam (Park et al., 2002)