Table 4.
Compatibility of high copy pSC101 vectors with the ColE1 replicon pBR322
Plasmid | Plasmid stability* | ||||
---|---|---|---|---|---|
Number of generations | |||||
1 | 20 | 40 | 60 | 80 | |
pWSK29-cam | 1.0 | 0.98 | 0.98 | 0.97 | 0.89 |
pJPC12 | 1.0 | 1.0 | 0.94 | 0.93 | 0.91 |
pJPC13 | 1.0 | 0.74 | 0.68 | 0.65 | 0.56 |
Plasmid stability reported as number of colonies appearing on LB + Cam/ number of colonies on LB + Amp (pBR322 selection) after growth for the indicated number of generations without antibiotics.