Figure 3.
Effects of iberin treatment on apoptosis induction in human neuroblastoma cells using FLICA/DAPI staining. A. The human neuroblastoma SK-N-AS cells were exposed to 25 μM of iberin for 24h. An equal volume of vehicle (DMSO) was added to the controls. To detect activation of caspases and chromatin condensation and fragmentation, cells were stained with FLICA and DAPI respectively as described in section 2 and subsequently apoptotic cells were quantified by fluorescent microscopy. B. Percentage of apoptotic cells are shown and each column represents the mean + SE of the data obtained from three independent experiments. Significant difference from the controls, *p<0.05; **p<0.01.