Figure 3. IL-10 inhibits LTD₄ induced gene expression.

Monocytes (A) and iDCs (B) were incubated overnight with or without IL-10 (20ng/ml) and stimulated with LTD₄ (100 nM) for one hour. In some experiments cells were exposed to MK571 (MK) (1 μM) for 5 minutes before stimulation with LTD₄. Expression of mRNA for Egr2 and FosB was measured by TaqMan. Results are normalized to an internal control (GAPDH) and presented as fold increase from control vehicle (ethanol) treated samples. The means ± SD from 3 different donors, done in triplicate, are shown. p < 0.001, in comparison to LTD₄ stimulated cells, by Student’s t-test.