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. Author manuscript; available in PMC: 2009 Jun 1.
Published in final edited form as: J Immunol. 2008 Jun 1;180(11):7597–7603. doi: 10.4049/jimmunol.180.11.7597

Figure 4. CysLT1 knockdown inhibits LTD4-induced activation of monocytes.

Figure 4

Monocytes were nucleofected with CysLT1 siRNA or negative control oligonucleotides (control siRNA) as described in the Methods section, cultured for 24 hours and CysLT1 mRNA was measured by TaqMan (A), calcium flux was measured in response to LTD4 (B) or LTD4-induced (100 nM) FosB mRNA expression was measured by TaqMan (C). TaqMan results are normalized to an internal control (GAPDH) and presented as fold increase from control (control siRNA) treated samples. The means ± SD from 3 donors, performed in triplicate are shown. * p < 0.001 in comparison to LTD4 stimulated control cells, by Student’s t-test.