Figure 3.

Construction of antisense PCDGF cDNA. A 228-bp PCDGF cDNA fragment (−45 to +183 bp) including the start codon region was ligated in the antisense orientation into the XbaI-SmaI site of pCMV₄ expression vector. Immediate early promoter region (CMV, stippled block), a DNA copy of a segment of the alfalfa mosaic virus 4 RNA that contains a translational enhancer (A), transcription termination and polyadenylation signals from the human growth hormone gene (hGH, gray block), and the simian virus 40 (SV40) origin of DNA replication and early region enhancer sequences (SV40_ori, white block) are shown. Locations of primers used for PCR analysis of transfectants are indicated.