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PCDGF protein expression in antisense and control transfected clones. PC cells and antisense and control transfectants were cultivated in DME/F12 medium supplemented with 2% FBS. The medium was replaced with fresh medium 24 hr before the samples were collected. Cell lysates and conditioned media were normalized by cell numbers of 18 × 105 and 3 × 105 cells, respectively, and were immunoprecipitated with anti-PCDGF IgG and analyzed by Western blot as described in Materials and Methods.
