Figure 7.

In vitro growth properties of antisense and control transfected clones. PC cells, antisense cDNA transfected cells, and empty vector control transfected cells were plated either in DME/F-12 medium supplemented with 2 μg/ml human fibronectin and 10 μg/ml human transferrin (2F medium, Lower) or with 2% FBS added 12 hr after plating (Upper). At day 5, cells from duplicate wells were trypsinized and counted with a Coulter counter. The experiment was repeated twice. Each bar represents the mean number of cells (±SD) calculated from both experiments.