Table 1.
Order-specific group primers and species-specific primers used in blood-meal identification
| Test animal | Primer sequence1 (F: forward 5'-3' and R: reverse 5'-3') | Annealing temp (°C) | Size of amplified products (bp) |
| Avian | F: GACTGTGACAAAATCCCNTTCCA R: GGTCTTCATCTYHGGYTTACAAGAC |
64 | 508 |
| Mammalian | F: CGAAGCTTGATATGAAAAACCATCGTTG R: TGTAGTTRTCWGGGTCHCCTA |
59 | 772 |
| Chicken | F: GGGACACCCTCCCCCTTAATGACA R: GGAGGGCTGGAAGAAGGAGTG |
69 | 266 |
| Bovine | F: GCCATATACTCTCCTTGGTGACA R: GTAGGCTTGGGAATAGTACGA |
61 | 271 |
| Pig | F: GCCTAAATCTCCCCTCAATGGTA R: ATGAAAGAGGCAAATAGATTTTCG |
64 | 212 |
| Goat | F: TTAAAGACTGAGAGCATGATA R: ATGAAAGAGGCAAATAGATTTTCG |
54 | 225 |
| Cat | F: TTCTCAGGATATACCCTTGACA R: GAAAGAGCCCATTGAGGAAATC |
60 | 180 |
| Dog | F: GAACTAGGTCAGCCCGGTACTT R: CGGAGCACCAATTATTAACGGC |
67 | 153 |
| Horse | F: CCCTAAGCCTCCTAATCCGT R: AGGAATGATGGGGGAAGTAA |
56 | 235 |
| Human | F: TTCGGCGCATGAGCTGGAGTCC R: TATGCGGGGAAACGCCATATCG | 70 | 228 |
| Monkey | F: CCTCTTTCCTGCTGCTAATG R: TTTGATACTGGGATATGGCG |
62 | 222 |
| Rat | F: CGGCCACCCAGAAGTGTACATC R: GGCTCGGGTGTCTACATCTAGG | 67 | 196 |
1The primer sequences were cited from papers of Ngo and Kramer [22], Lahiff et al [23] and Parodi et al [24].