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. 2008 May 22;27(12):1658–1670. doi: 10.1038/emboj.2008.103

Figure 8.

Figure 8

Endogenous VE-statin/egfl7 inhibits elastic fibre deposition by repressing lysyl oxidase activity in HUVEC cells. (A) Measure of lysyl oxidase enzymatic activity in control- (mock) or VE-statin/egfl7-siRNA (siRNA VE-statin)-transfected HUVEC cells. Each experiment was performed in triplicate. Results are expressed as mean±s.d. *P<0.05. (B) VE-statin/egfl7 is immunodetected (top left panel), whereas elastic fibres are not observed (top central panel) in siRNA control (siRNA ctrl)-transfected HUVEC cells. After VE-statin/egfl7 siRNA transfection, VE-statin/egfl7 is no longer detected (bottom left panel), whereas mature elastic fibres are abundantly deposited by HUVEC cells (bottom central panel). Right panel: merged images of the same field. (C) After co-transfection of control siRNA and VE-statin/egfl7 siRNA (siRNA ctrl/si RNA VE-statin), LoxL2 and mature elastin are detected by immunofluorescence in HUVEC cells. Co-transfection of LoxL2-siRNA and VE-statin/egfl7-siRNAs (siRNA LoxL2/siRNA VE-statin) in HUVEC cells prevents elastic fibre formation.