Kinetics of the enzyme-catalysed branch migration reaction and effect of heterologies. (A) Product formation versus time for single-molecule experiments measured as the number of molecules that completed the reaction at a given time, normalized by the total number of molecules that complete the reaction and (B) bulk solution experiments. (C) Effect of heterologies on branch migration reactions. Histograms of the times that molecules spend in the pre-high (left panel), high (central panel), and low (right panel) FRET states, indicated in orange, blue, and red, respectively (each stage is also indicated in Figure 1E). The results for HomoJ, HomoJ/het5, and HomoJ/het16/het17 (from top to bottom) are shown. Roman numbers on the left indicate substrate schematics in Figure 3A.