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. 2008 May 22;27(12):1694–1705. doi: 10.1038/emboj.2008.94

Figure 3.

Figure 3

Nuclear retention of IRAlus-containing RNAs correlates with silencing of EGFP expression. RNA in situ hybridization (A, E, I, M) was performed with Dig-labelled antisense egfp probe (red) for each different transfection with plasmids encoding either IRAlus or Alu-RNA, and representative images are shown. No signals were detected with the Dig-labelled sense strand egfp fragment (data not shown). EGFP was visualized using anti-GFP antibodies (B, F, J, N). The white arrows in panel A identify cells in which mRNA is in either the cytoplasm or nucleus. Note that when RNA expression in the cytoplasm is higher, in these cells the expression of GFP is also higher. When the RNA is retained in the nucleus, GFP expression is reduced. Panels C, G, K and O merge the RNA and GFP signals, and panels D, H, L and P merge the RNA signal with nuclear DAPI staining. Scale bars 10 μm.