. 2008 May 22;27(12):1706–1717. doi: 10.1038/emboj.2008.99

Table 5.

Effects of mRNA and tRNA identity on the splitting of ribosomal complexes by RRF/EF-G and IF1/IF3

Splitting by RRF/EF-G	A₁ (%)	q₁ (s⁻¹)	A₂ (%)	q₂ (s⁻¹)
70S:mRNA:tRNA^Phe with
mXR7	85	1.77±0.06	15	0.33±0.03
mXR8	81	1.72±0.06	19	0.32±0.03
mXR8_FM	78	1.44±0.05	22	0.33±0.04
mBar	55	2.8±0.08	45	0.31±0.05
mSD022	65	1.47±0.06	35	0.29±0.04

70S:mRNA:tRNA^fMet with
mXR7	88	0.51±0.02	12	0.12±0.03
mXR8	85	0.48±0.02	15	0.13±0.02
mXR8_FM	47	0.47±0.05	53	0.18±0.03
mBar	73	0.48±0.03	27	0.11±0.02
mSD022	71	0.51±0.03	29	0.15±0.03

70S:mRNA:tRNA^fMet prepared from initiation complexes with
mXR7	92	0.54±0.02	8	0.09±0.02
mXR8	86	0.51±0.02	14	0.08±0.02
mXR8_FM	55	0.41±0.04	45	0.08±0.03
mBar	64	0.50±0.04	36	0.11±0.03
mSD022	81	0.48±0.03	19	0.09±0.02

Splitting by IF1/IF3	A₁	q₁ (s⁻¹)	A₂	q₂ (s⁻¹)
70S	81	0.07±0.03	19	0.03±0.005
70S:mXR7	87	1.23±0.06	13	0.08±0.02
70S:mXR8	83	0.81±0.05	17	0.07±0.01
70S:mXR8_FM	76	1.05±0.06	24	0.06±0.01
70S:mBar	59	0.28±0.04	41	0.05±0.02
70S:mSD022	80	0.11±0.03	20	0.04±0.005
Parameters A₁, A₂, q₁ and q₂ were determined from nonlinear regression fit of experimental data to the relation $I(t) = A_{1} exp ( - q_{1} t) + A_{2} exp ( - q_{2} t) + A_{{{rm BKG}}} $ (Equation (10) in Materials and methods). All experiments were conducted at 37°C in LS3 buffer containing 3 mM Mg²⁺.