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. 2008 Jun 20;14:1138–1148.

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This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Validation of three genes differentially expressed in human retinal pericytes (hRPC) in response to hypoxia. The upregulation of a selection of genes was validated with real time PCR, using a PerkinElmer 7700 analyzer, on cDNA generated from human retinal pericytes exposed to increasing periods of hypoxia (0, 6, 24, and 48 h). All results were normalized to 18S rRNA, using a pre-developed assay reagent. Data are expressed as mean relative quantity of mRNA, relative to control, for three independent experiments ±standard error of measurement for (A) CHL-1 mRNA, (B) VEGF mRNA, and (C) Cox 2 mRNA. Data are expressed as mean±SEM values. The asterisk indicates a significance at p<0.05 and the double asterisk indicates a significance at p<0.001.