Table 1. Limited cycle and real time PCR primers.

Limited cycle PCR primers
Gene	Sense primer 5′-3′	Antisense primer 5′-3′	Probe 5′-3′
VEGF	CTGCTCTACCTCCACCATGC	CTGCATTCACATTTGTTGTGC
CHL-1	TCTGTCACCTTTTGCAGTGG	GTTGCCGATTCTGAA AGAGC
Cox2	AACAGGAGCATCCTGAATGG	ATTTCATCTGCCTGCTCTGG
RTP801	TCTTAGCAGTTCTCGCTGACC	TGGCACACAAGTGTTCATCC
BMP-2	CAAGATGAACACAGCTGG	TTTGCTGTACTAGCGACACC
BMP-4	GGCTGTCAAGAATCATGG	GCTCAGGATACTCAAGAC
18S	GTGGAGCGATTTGTCTGGTT	CGCTGAGCCAGTCAGTGTAG
Real time PCR primers
Gene	Sense primer 5′-3′	Antisense primer 5′-3′	Probe 5′-3′
VEGF	GTGCCCACTGAGGAGTCCA	GTGCTGGCCTTGGTGAGGT	CATCACCATGCAGATTATGCGGATCAA
Cox2	AGCCCTTCCTCCTGTGCCT	CAGGAAGCTGCTTTTTACC	TGATTGCCCGACTCCCTTG
CHL-1	CGTAGCTGAAGGGCTCTTT	ACACGTTTCCCTCCGAACA	AAATCGGCAACCCAATCAAT

Limited cycle PCR was performed using primers, designed using Primer3 software [11]. For real-time, PCR probes were labeled with 5′-FAM and 3′-TAMRA as quencher with the exception of the ribosomal probe, which was labeled with 5′-VIC to facilitate multiplexing.