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. Author manuscript; available in PMC: 2008 Jun 21.
Published in final edited form as: J Bone Miner Res. 2006 Apr 5;21(4):637–646. doi: 10.1359/JBMR.060109

FIG. 1.

FIG. 1

Autocrine BMP synthesis in C3H10T1/2 cells. Cells were transduced with AdLacZ (open bars) or AdRUNX2 (gray bars) at 100 pfu/cell and harvested after 6 days for analysis of BMP (A) mRNA and (B) protein. Relative mRNA levels were measured by Q-RT/PCR and normalized to GAPDH mRNA. BMP protein in whole cell extracts was measured on Western blots. BMP-2, -4, and -7 standards (STD) were prepared from COS7 cell-conditioned medium.