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. Author manuscript; available in PMC: 2008 Jun 21.
Published in final edited form as: J Bone Miner Res. 2006 Apr 5;21(4):637–646. doi: 10.1359/JBMR.060109

FIG. 4.

FIG. 4

Effect of BMP inhibition on AA-induced osteoblast differentiation markers in MC3T3-E1 pre-osteoblast cells. (A) Clone 4 MC3T3-E1 cells were growth in the presence (gray bars) or absence of AA (open bars) and treated with combined BMP2/4 and BMP7 blocking antibodies (5 ηg/ml each) for 5 days and assayed for ALP activity. Total RNA was isolated from replicate samples and assayed for (B) OCN, (C) BSP, and (D) RUNX2 mRNA using Q-RT/PCR.