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Western blot analysis of BMP2-responsive signal transduction pathways. C3H10T1/2 cells were transduced with Ad-LacZ or AdRUNX2 at 100 pfu/cell. Forty-eight hours after transduction, cells were treated with rhBMP2 for 30 minutes, harvested, and assayed on Western blots for the following signaling intermediates: phospho-SMAD 1/5/8, total SMADs 1/5, phospho-JNK, total JNK, phospho-p38, total p38, phospho-ERK, total ERK, and total RUNX2.
