Table 5.

Analysis of putative transcription factor binding sites lost or gained by carriage of alternative alleles at SNPs in the upstream regions of LECT2 and TGFBI that show allelic association with the DTH+ phenotype.

SNP	Allele	TFBS	Description	Tool
rs248158_L	G	CEBPA C/EBPα	CCAAT enhancer binding protein, alpha; myeloid TF crucial for granulopoiesis	AliBaba
rs2526146_M	G	CEBPB C/EBPβ	NF-IL6, nuclear factor triggered by interleukin 6	PROMO
rs2526146_M	G	c-Jun	Component of AP1 TF	PROMO
rs2526146_M	G	LXRE	Highly conserved DR1 element selected by LXRbeta/RXR heterodimers	MatInspector
rs2526146_M	G	LEF1	Lymphoid enhancer-binding factor-1 expressed in pre-B and T cells, involved in the Wnt signal transduction pathway	MatInspector
rs2526152_N	A	EVI1	Ecotropic viral integration site 1 encoded factor, amino-terminal zinc finger domain; myeloid TF	MatInspector
rs2526152_N	A	YY1	Yin and Yang 1 ubiquitous TF	MatInspector
rs2526152_N	A	SMARCA3	SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 3	MatInspector
rs4976360_R	T	HSF	Heat shock TF	AliBaba
rs4976360_R	T	E2F	Critical determinant of G1/S-phase transition during the mammalian cell cycle	MatInspector
rs4976360_R	A	CEBPA C/EBPα	CCAAT enhancer binding protein, alpha; myeloid TF crucial for granulopoiesis	AliBaba
rs4976360_R	A	STAT3	TF for acute phase response gene	MatInspector

Under allele, bold indicates the DTH+ associated allele, normal type indicates the allele not associated with the DTH response. Bioinformatic analysis demonstrated that the transcription factor binding site (TFBS) was only present with the allele indicated, and was lost with the alternative allele at each SNP shown. TF = transcription factor.