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. Author manuscript; available in PMC: 2009 Mar 1.
Published in final edited form as: DNA Repair (Amst). 2008 Jan 22;7(3):464–475. doi: 10.1016/j.dnarep.2007.12.003

Fig. 8.

Fig. 8

Autophosphorylation of ATM is insufficient for prevention of DNA end-degradation. (A) Top Strand primer extension products following gel separation. The 5′AATTC substrate was first incubated with WI-38VA13 (C) or AT5BIVA (A) nuclear extracts. Pre-phosphorylated ATM, wortmannin (W), caffeine (C) and the phosphatase inhibitor fostriecin were added where indicated. Products were isolated and then subjected to a primer extension assay. (B) Autoradiogram of 32P-ATM incubated with AT5BIVA (A-T NE) nuclear extract, wortmannin and a duplex with a 5′AATTC overhang under repair reaction conditions with or without fostriecin. 32P-ATM was monitored in parallel with reactions in Fig. 7A to ensure that pre-phosphorylated ATM remained phosphorylated in the reactions.