Table 2.
Percent intensity of end-processing assay products using a 5′AATTC substrate
| Nuclear extract
|
Duplex only | Primer only | ||||
|---|---|---|---|---|---|---|
| Control
|
A-T
|
|||||
| WI-38VA13 | GM16667 | AT5BIVA | GM16666 | |||
| (A) Top Strand primer extension products | ||||||
| Product | ||||||
| Full-length | 22 | 13 | 1 | 1 | 44 | 2 |
| Long | 6 | 8 | 0 | 2 | 5 | 5 |
| Medium | 6 | 13 | 13 | 0 | 0 | 0 |
| Short | 9 | 19 | 10 | 2 | 0 | 10 |
| Un-extended primer | 57 | 47 | 76 | 95 | 51 | 83 |
| Lane (Fig. 2A) | 1 | 2 | 3 | 4 | 5 | 6 |
| Nuclear extract
|
Duplex only | |||||
| Control WI-38VA13 | A-T AT5BIVA | |||||
|
| ||||||
| (B) 5′Cy3 Template degradation products | ||||||
| Product | ||||||
| Full-length | 73 | 9 | 93 | |||
| Long | 12 | 42 | 7 | |||
| Medium | 15 | 38 | 0 | |||
| Short | 0 | 11 | 0 | |||
| Lane (Fig. 2B) | 1 | 2 | 3 | |||