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Percent intensity of primer extension products of the Top Strand in a 5′AATTC substrate in reactions with or without ATP
| Nuclear extract
|
Duplex only
|
|||||
|---|---|---|---|---|---|---|
| Control WI-38VA13
|
A-T AT5BIVA
|
− | + | |||
| ATP | − | + | − | + | ||
| Product | ||||||
| Full-length | 1 ± 1 | 18 ± 5 | 3 ± 1 | 1 ± 1 | 17 ± 3 | 18 ± 3 |
| Long | 3 ± 2 | 5 ± 4 | 3 ± 3 | 4 ± 4 | 6 ± 1 | 6 ± 2 |
| Medium | 4 ± 4 | 2 ± 3 | 0 ± 3 | 3 ± 3 | 4 ± 1 | 4 ± 3 |
| Short | 0 ± 0 | 0 ± 1 | 0 ± 0 | 3 ± 6 | 0 ± 0 | 0 ± 0 |
| Un-extended primer | 92 ± 5 | 75 ± 5 | 94 ± 1 | 89 ± 5 | 73 ± 4 | 72 ± 7 |
