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. Author manuscript; available in PMC: 2009 Mar 1.
Published in final edited form as: Mol Cancer Res. 2008 Mar;6(3):501–508. doi: 10.1158/1541-7786.MCR-07-2075

FIGURE 3.

FIGURE 3

MKK4 mRNA is primarily nuclear. PC3 and LNCaP cells were fractionated into nuclear and cytoplasmic components, and RNA and protein samples were prepared. Total RNA (5 μg) was used for Northern blot analysis to detect MKK4 and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) mRNAs. Protein (20 μg) was used for immunoblotting for the nuclear markers lamin A and C, and the cytoplasmic marker α-tubulin. Data are representative of three independent experiments.