Table 2.
Statistical analysis of responses of sphingolipids to PDT in scrambled siRNA- and SMS1 siRNA-transfected cells.
| FCG | FCD | pGroupFDR | pDoseFDR | |
|---|---|---|---|---|
| C26Ceramide | 1.74 | 1.21 | 6.4E-08 | 2.39E-06 |
| C24Ceramide | 1.35 | 0.99 | 1.2E-05 | 5.20E-01 |
| C24:1Ceramide | 1.36 | 1.05 | 2.7E-04 | 1.07E-01 |
| C22Ceramide | 1.56 | 1.10 | 2.7E-04 | 4.86E-02 |
| C26:1Ceramide | 1.45 | 1.48 | 1.0E-03 | 4.71E-09 |
| C16Ceramide | 1.16 | 1.27 | 7.0E-02 | 6.13E-08 |
| C22:1Ceramide | 1.34 | 2.02 | 7.1E-02 | 4.34E-10 |
| C20Ceramide | 1.33 | 1.78 | 7.1E-02 | 8.76E-09 |
| C18:1Ceramide | 1.20 | 1.47 | 8.2E-02 | 1.00E-08 |
| C20:1Ceramide | 1.26 | 1.97 | 2.7E-01 | 3.84E-07 |
| C18Ceramide | 1.10 | 1.93 | 3.6E-01 | 2.17E-12 |
| C14Ceramide | 1.00 | 1.50 | 9.6E-01 | 3.50E-11 |
| DHC24:1-Cer | 1.67 | 1.77 | 2.2E-02 | 8.71E-08 |
| DHC24-Cer | 1.40 | 1.23 | 2.2E-02 | 4.42E-04 |
| DHC22-Cer | 1.27 | 1.32 | 2.2E-02 | 1.20E-07 |
| DHC22:1-Cer | 1.39 | 1.25 | 1.6E-01 | 8.16E-03 |
| DHC14-Cer | 1.23 | 2.36 | 2.6E-01 | 2.18E-12 |
| DHC16-Cer | 1.27 | 2.17 | 2.6E-01 | 4.40E-09 |
| DHC20:1-Cer | 1.25 | 1.78 | 2.6E-01 | 1.08E-06 |
| DHC18:1-Cer | 0.81 | 1.70 | 2.6E-01 | 1.12E-07 |
| DHC18-Cer | 1.16 | 3.98 | 4.8E-01 | 2.71E-13 |
| DHC26-Cer | 1.08 | 1.13 | 5.4E-01 | 1.98E-02 |
| DHC26:1-Cer | 1.05 | 1.45 | 8.1E-01 | 1.11E-05 |
| DHC20-Cer | 1.01 | 1.89 | 9.7E-01 | 4.36E-05 |
| C14-SM | 1.33 | 0.93 | 3.4E-02 | 4.07E-01 |
| C20:1-SM | 0.83 | 1.00 | 6.3E-02 | 9.57E-01 |
| C16-SM | 1.20 | 0.97 | 6.3E-02 | 7.09E-01 |
| C18-SM | 1.16 | 0.95 | 1.1E-01 | 5.31E-01 |
| C26:1-SM | 1.26 | 0.95 | 1.1E-01 | 6.14E-01 |
| C18:1-SM | 1.24 | 1.02 | 1.2E-01 | 8.87E-01 |
| C22-SM | 0.92 | 0.94 | 4.3E-01 | 4.07E-01 |
| C20-SM | 1.09 | 1.01 | 4.4E-01 | 8.87E-01 |
| C24:1-SM | 0.94 | 0.98 | 4.4E-01 | 7.70E-01 |
| C22:1-SM | 0.97 | 0.96 | 8.6E-01 | 5.76E-01 |
| C24-SM | 0.99 | 0.95 | 9.7E-01 | 4.07E-01 |
| C26-SM | 1.00 | 1.00 | 9.7E-01 | 9.57E-01 |
| Sph | 2.35 | 1.05 | 4.4E-04 | 6.03E-01 |
| Sph-1P | 0.54 | 1.28 | 2.6E-02 | 4.72E-02 |
| DHSph-1P | 0.75 | 1.64 | 3.6E-01 | 2.63E-03 |
| DHSph | 1.22 | 1.64 | 3.6E-01 | 1.35E-04 |
DHC24:1-Cer, C24:1-dihydroceramide; C14-SM, C14-sphingomyelin. Similar abbreviations were used for other dihydroceramides and sphingomyelins, respectively. Sph, sphingosine; Sph-1P, sphingosine-1-phosphate, DHSph-1P, dihydrosphingosine-1-phosphate; DHSph, dihydrosphingosine.
The FCG is the estimated fold-change between cell lines (groups) regarding a sphingolipid response, assuming a constant PDT dose. The FCD is the estimated fold-change in response to each 100 units of dose (light fluence), regardless of the group. The pGroupFDR and the pDoseFDR are corresponding adjusted p-values using false discovery rate algorithm. Bold type indicates statistical significance.