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. Author manuscript; available in PMC: 2008 Aug 1.
Published in final edited form as: Cancer Biol Ther. 2008 Apr 24;7(7):1109–1115. doi: 10.4161/cbt.7.7.6192

Figure 5.

Figure 5

Sustained IFN-λ induced STAT activation requires de novo protein synthesis (A) HaCaT cells were stimulated with IFN-α (5 ng/mL) or IFN-λ (100 ng/mL) for 30 min followed by pulse chase with staurosporine (STS) for the indicated times. Cell extracts were prepared and immunoblot analysis was performed with antibodies against pY-STAT1, pY-STAT2, total STAT1, total STAT2. β-actin served as an internal control for equal loading (B) Same as in (A) except, cells were pretreated with cycloheximide (5μg/mL) followed with IFN-α or IFN-λ treatment.