Figure 10.

15d-PGJ₂ is a global inhibitor of astrocytic IL-12 p40 release following TLR ligand exposure. Primary astrocytes were plated at 1 × 10⁵ cells/well in 96-well plates and incubated overnight. The following day, cells were pretreated for 1 hour with the indicated concentrations of 15d-PGJ₂ prior to the addition of TLR ligands. Cell-conditioned supernatants were collected at 24 hours following TLR ligand exposure, whereupon IL-12 p40 levels were determined by ELISA (a) and (b). Significant differences between astrocytes treated with TLR ligands alone versus TLR ligands + 15d-PGJ₂ are noted with asterisks (*P < .05;**P < .001). Results presented are representative of two independent experiments.