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hLsm1 overexpression induces growth in soft agar. MCF10Lsm1 – insulin cells, MCF10LacZ cells and SUM44 cells were plated in soft agar and given appropriate medium. After 3 weeks, medium was removed and 1 mg/ml of p-iodonitrotetrazolium violet was added overnight to stain viable cells. All MCF10Lsm1 and MCF10LacZ clones were evaluated and representative photographs of colony formation are shown.
