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. Author manuscript; available in PMC: 2008 Jun 22.
Published in final edited form as: Oncogene. 2006 Sep 25;26(14):2104–2114. doi: 10.1038/sj.onc.1210002

Figure 4.

Figure 4

hLsm1 siRNA reduces hLsm1 mRNA and protein expression in SUM44cells. hLsm1 or control siRNA oligonucleotides (100 nmol/l) were transfected into the SUM44 cells using RNAifect. RNA or whole-cell lysates were collected 1, 2 and 3 days post transfection. (a) hLsm1 mRNA levels were measured by Q-RT-PCR in two separate reactions, each in triplicate. Cycles to threshold values for hLsm1 were normalized to values for GAPDH, then compared to MCF10A hLsm1 expression to determine fold change. (b) Protein levels of hLsm1 were measured by Western blot, quantified with Quantity-One software and compared to the protein expression of β-actin (c). Experiments were repeated at least twice and data are shown as means±standard error.