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hLsm1 siRNA reduces soft agar colony formation in SUM44 cells. (a) SUM44, SUM44+ control siRNA or SUM44+ hLsm1 siRNA cells were plated into soft agar 24 h after transfection. After 3 weeks, medium was removed and p-iodonitrotetrazolium violet (1 mg/ml) was added overnight to stain viable cells. (b) Colonies were counted in triplicate wells of each cell type using Quantity-One software. Experiments were repeated at least twice and data are shown as means±standard error.
