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. Author manuscript; available in PMC: 2008 Jun 23.
Published in final edited form as: J Microbiol Methods. 2007 Dec 15;72(3):249–256. doi: 10.1016/j.mimet.2007.12.002

Fig. 1.

Fig. 1

Schematic diagrams of fusion constructs. pVT1666 (A) was used as a backbone to generate fusion vectors pVPT-CTag and pVPT-NTag (B) with five tags fused to Gtf2 at either N-terminus or C-terminus (C).PM, maltose promoter; CTag (CGST, CHSV, CNano or CT7) stands for GST, HSV, Nano or T7 tag fused to the C-terminus of Gtf2 protein by using SalI and KpnI sites. NTag (NGFP, NGST, NHSV, NNano or NT7) stands for GFP, GST, HSV, Nano or T7 tag fused to the N-terminus of Gtf2 protein by using BamHI site. Start codon (ATG) and stop codon (TGA) are indicated with grey rectangle and black rectangle, respectively.