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. Author manuscript; available in PMC: 2008 Jun 23.
Published in final edited form as: J Microbiol Methods. 2007 Dec 15;72(3):249–256. doi: 10.1016/j.mimet.2007.12.002

Fig. 2.

Fig. 2

Immunodetection of fusion constructs. (A) Western blot analyses of expression of fusion tags in E. coli. Lane 1, Top10; lane 2, Top10/pVPT-CTag; lane 3, Top10/pVPT-Gtf2-CTag; lane 4, Top10/pVPT-NTag-Gtf2. (B) Western blot analyses of expression of fusion tags in S. parasanguinis. Lane 1, wild type; lane 2, gtf2−; lane 3, gtf2−/pVPT-CTag; lane 4, gtf2−/pVPT-Gtf2-CTag; lane 5, gtf2−/pVPT-NTag-Gtf2. Arrows point to the position corresponding to tag or tagged Gtf2 fusion proteins.