Table 1.
Strains and plasmids used in this study
| Strains | Relevant properties | Source |
|---|---|---|
| E. coli | ||
| Top10 | for propagation of the recombinant plasmids | Invitrogen |
| S. parasanguinis | ||
| FW213 | wild type | Cole et al., 1976 |
| fap1− | wild type; fap1::aphA3 | Chen et al., 2002 |
| gtf2− | Wild type; gtf2::aphA3 | Wu et al. unpublished |
| S. mutans UA159 | wild type | Ajdiæ et al., 2002 |
| S. pneumoniae V047 | wild type | Hollingshead |
| S. sanguinis SK36 | wild type | Xu et al., 2007 |
| Plasmids | ||
| pVT1666 | GFP tag for C-terminal fusion | Chen et al., 2006 |
| PGEX-6P-1 | GST fusion vector | Invitrogen |
| pET27b(+) | HSV tag expression vector | Novagen |
| pVPT-NGFP | GFP tag for N-terminal fusion | This study |
| pVPT-CGST | GST tag for C-terminal fusion | This study |
| pVPT-NGST | GST tag for N-terminal fusion | This study |
| pVPT-CHSV | HSV tag for C-terminal fusion | This study |
| pVPT-NHSV | HSV tag for N-terminal fusion | This study |
| pVPT-Cnano | Nano tag for C-terminal fusion | This study |
| pVPT-Nnano | Nano tag for N-terminal fusion | This study |
| pVPT-CT7 | T7 tag for C-terminal fusion | This study |
| pVPT-NT7 | T7 tag for N-terminal fusion | This study |
| pVPT-Gtf2-CGFP | gtf2 cloned in pVT1666 | This study |
| pVPT-NGFP-Gtf2 | gtf2 cloned in pVPT-NGFP | This study |
| pVPT-Gtf2-CGST | gtf2 cloned in pVPT-CGST | This study |
| pVPT-NGST-Gtf2 | gtf2 cloned in pVPT-NGST | This study |
| pVPT-Gtf2-CHSV | gtf2 cloned in pVPT-CHSV | This study |
| pVPT-NHSV-Gtf2 | gtf2 cloned in pVPT-NHSV | This study |
| pVPT-Gtf2-Cnano | gtf2 cloned in pVPT-Cnano | This study |
| pVPT-NNano-Gtf2 | gtf2 cloned in pVPT-Nnano | This study |
| pVPT-Gtf2-CT7 | gtf2 cloned in pVPT-CT7 | This study |
| pVPT-NT7-Gtf2 | gtf2 cloned in pVPT-NT7 | This study |