Fig. 4.

Western blot analysis of ERα, ERβ, SRC-2 and SRC-3 in the aortic tissue extracts from WT and SRC-1^−/− (KO) mice. The β-actin serves as a loading control. The tissues extracts were prepared by pooling the aortic samples of 4 mice. The assays in panel A used the first batch of samples. The assays in panels B-D used the second batch of samples, so the β-actin bands in these three panels were the same. The relative amount of ERα, ERβ, SRC-2 and SRC-3 proteins in WT and SRC-1^−/− aortic samples was quantitatively reflected by the ratios of individual band intensities of these proteins to the band intensity of corresponding β-actin. The ratio for WT samples was set as 1.