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. 2008 Jul 4;4(7):e1000095. doi: 10.1371/journal.ppat.1000095

Figure 3. CEM-T4 cells lack an A3G/A3F cofactor.

Figure 3

(A) A schematic description for transient trans-complementation assay. T cells were infected with env-defective HIV-1 virus pseudotyped with VSV-G, and 293T cells were transfected with the Env-expressing vector pNLΔGag. These two types of cells were cocultured for heterokaryon formation. Infectious particles were then detected by infection of TZM-bI cells. (B) Infectivity of HIV-1 produced from heterokaryons. 293T cells were transfected with either pNLΔGag or pNLΔGagΔVif in the presence or absence of a human A3G or A3F expression vector and then cocultured with A2.01, A3.01, HUT 78, H9, CEM-SS, or CEM-T4 T cells that were infected with VSV-G–pseudotyped env-defective HIV-1 expressing or not expressing Vif protein. Infectious particles were collected 48 h later and used to infect TZM-bI cells. Viral infectivity was finally determined by measuring firefly luciferase activity in TZM-bI cell lysates. Results shown here were from 1 of 3 independent experiments.