Skip to main content
. 2008 Jul 4;4(7):e1000095. doi: 10.1371/journal.ppat.1000095

Figure 4. Anti-HIV activity of A3F/A3G proteins expressed in cis from an HIV-based vector.

Figure 4

(A) Expression of A3G/A3F from an HIV-based vector and the antiviral infectivity in a single round replication assay. An A3F, A3G, or A3GE259Q gene with an HA tag was inserted into the Nef open reading frame in pNL4-3 or pNL4-3ΔVif. These constructs were then cotransfected into 293T cells with either pNL-A1 or its control pNL-A1ΔVif as indicated. Expressions of Vif and A3 proteins in these transfections were determined by Western blotting, and viral infectivity was determined in TZM-bI cells. (B) Replication kinetics of HIV-1 viruses expressing A3G or A3F. CEM-SS and CEM-T4 cells were infected with the same amount of A3G- or A3F-expressing HIV-1 viruses used as in (A); viral growth curves were determined by measuring p24Gag in the supernatants. pNLA3FΔVif + Vif, pNLA3GΔVif + Vif, and pNLA3GE259QΔVif + Vif are viruses produced in the presence of pNL-A1 during transfection of 293T cells.