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. Author manuscript; available in PMC: 2008 Jun 23.
Published in final edited form as: Biochemistry. 2007 Feb 13;46(6):1663–1671. doi: 10.1021/bi0617613

FIGURE 2.

FIGURE 2

DNase I footprinting analysis on expressed wt bZIP bound to AP-1, C/EBP, XRE1, E-box, HRE, and Partial full sites, as well as cognate and E-box half sites. Data presented for 5′ 32P-endlabeled DNA (∼20000 cpm/lane). Lanes 1, 5, 9, 13, 17, and 21: chemical sequencing G reactions. Lanes 2, 6, 10, 14, 18, and 22: DNase I cleavage control reactions. Lanes 3, 7, 11, and 15: DNase I cleavage reactions with 0.5 μM wt bZIP. Lanes 19 and 23: DNase I cleavage reactions with 1 μM wt bZIP. Lanes 4, 8, 12, 16, 20, and 24: DNase I cleavage reactions with 3 μM wt bZIP. The positions of the corresponding DNA targets are indicated. A indicates footprints at cognate half site TGAC, and B indicates footprints at E-box half site CAC. The results were reproduced at least three times.