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. Author manuscript; available in PMC: 2008 Jun 23.
Published in final edited form as: Biochemistry. 2007 Feb 13;46(6):1663–1671. doi: 10.1021/bi0617613

FIGURE 3.

FIGURE 3

EMSA on synthetic wt bZIP bound to all full and half sites. Each lane contains ∼3000 cpm 32P-endlabeled DNA 24-mer duplex (full sites) or 20-mer duplex (half sites). I indicates free DNA; II indicates a band shift from dimeric wt bZIP complexation. Parts A and B represent different EMSA gels. (A) EMSA on synthetic wt bZIP bound to full sites. Lane 1, free AP-1 DNA; lanes 2-11, DNA in the presence of 1500 nM synthetic wt bZIP. DNA target sites used in lanes 2-11 are WT AP-1, AP-1, CRE, C/EBP, XRE1, Arnt E-box, Max E-box, HRE, Partial site, and nonspecific control, respectively. (B) EMSA on synthetic wt bZIP bound to half sites. Lane 1, free AP-1 half site DNA; lanes 2-5, DNA in the presence of 1500 nM synthetic wt bZIP. DNA target sites used in lanes 2-5 are the half sites of AP-1, C/EBP, Arnt E-box, and Max E-box, respectively.