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. Author manuscript; available in PMC: 2008 Jun 23.
Published in final edited form as: Biochemistry. 2007 Feb 13;46(6):1663–1671. doi: 10.1021/bi0617613

FIGURE 5.

FIGURE 5

CD on synthetic wt bZIP in the presence of DNA duplexes: synthetic wt bZIP control (○), C/EBP (△), Arnt E-box (□), nonspecific control (···), and AP-1 (-). Protein dimer was placed in 200 μL of 12.5 mM potassium phosphate buffer (pH 7.0), 50 mM KCl, such that final concentrations of protein dimer and DNA duplex were 2.5 μM each. Samples were equilibrated for 5 min at 15 °C prior to measurement. Each spectrum was averaged five times, and curves were not subjected to smoothing. The DNA control spectrum (including buffer) was subtracted from each protein-DNA spectrum.