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. Author manuscript; available in PMC: 2008 Jun 23.
Published in final edited form as: J Proteome Res. 2007 Jun 13;6(7):2786–2791. doi: 10.1021/pr070013c

Figure 2.

Figure 2

Evaluation of NF-H dephosphorylation by Western blotting. Triton-insoluble neurofilament extracts were prepared from lumbar sections, according to the procedure described in the Experimental Section. Individual aliquots of each preparation were subjected to isoelectric focusing followed by electrophoresis on 6% SDS–PAGE gels. Western blotting using the SMI-32 IgG antibody specific for DeNF-H was used to evaluate the control (A) and EAE (B) tissues.