Table 1.
RE | 11-cis-RAL | all-trans-RAL | all-trans-ROL | |
---|---|---|---|---|
Dark | ||||
Control | 4.63 ± 0.71 | 2.82 ± 0.18 | 0.46 ± 0.03 | 0.04 ± 0.01 |
MO | 4.5 ± 0.72 | 1.52 ± 0.08 | 0.18 ± 0.01 | 0.08 ± 0.01 |
Bleached | ||||
Control | 6.6 ± 1.19 | 1.65 ± 0.02 | 0.34 ± 0.01 | 0.11 ± 0.01 |
MO | 6.09 ± 1.08 | 0.53 ± 0.01 | 0.08 ± 0.01 | 0.06 ± 0.01 |
Bleached/dark | ||||
Control | 6.28 ± 1.42 | 2.81 ± 0.18 | 0.36 ± 0.02 | 0.01 ± 0.01 |
MO | 5.27 ± 1.14 | 1.40 ± 0.07 | 0.40 ± 0.02 | 0.11 ± 0.01 |
Retinoids were extracted from 50 larval heads under dim red light and subjected to HPLC analysis. Values (pmol per eye pair) are the mean ± SD of three independent experiments. Dark, dark-adapted overnight; bleached, dark-adapted overnight and illuminated with 10 000 lux for 20 min; bleached/dark, dark-adapted overnight and illuminated with 10 000 lux for 20 min and dark-readapted for 45 min. Control, WT larvae; MO, RPE65a-knockdown larvae.