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. Author manuscript; available in PMC: 2008 Jun 23.
Published in final edited form as: Eur J Neurosci. 2007 Sep 14;26(7):1940–1949. doi: 10.1111/j.1460-9568.2007.05801.x

Table 1.

Retinoid composition of the eyes of 5-dpf larvae subjected to different light conditions

RE 11-cis-RAL all-trans-RAL all-trans-ROL
Dark
 Control 4.63 ± 0.71 2.82 ± 0.18 0.46 ± 0.03 0.04 ± 0.01
 MO 4.5 ± 0.72 1.52 ± 0.08 0.18 ± 0.01 0.08 ± 0.01
Bleached
 Control 6.6 ± 1.19 1.65 ± 0.02 0.34 ± 0.01 0.11 ± 0.01
 MO 6.09 ± 1.08 0.53 ± 0.01 0.08 ± 0.01 0.06 ± 0.01
Bleached/dark
 Control 6.28 ± 1.42 2.81 ± 0.18 0.36 ± 0.02 0.01 ± 0.01
 MO 5.27 ± 1.14 1.40 ± 0.07 0.40 ± 0.02 0.11 ± 0.01

Retinoids were extracted from 50 larval heads under dim red light and subjected to HPLC analysis. Values (pmol per eye pair) are the mean ± SD of three independent experiments. Dark, dark-adapted overnight; bleached, dark-adapted overnight and illuminated with 10 000 lux for 20 min; bleached/dark, dark-adapted overnight and illuminated with 10 000 lux for 20 min and dark-readapted for 45 min. Control, WT larvae; MO, RPE65a-knockdown larvae.