Expression of ETRB2 and ETRB by quail NCC cultured in the absence (Left) and presence (Right) of ET3. In situ hybridization with ETRB2 (A, B, E, and F) and ETRB (C, D, G, and H) dig-labeled riboprobes. (A–D) At d3 ET3 promotes increase in ETRB2 (A and B) and ETRB (C and D) transcripts. (E–H) At d6, ET3-treated cultures show enhanced expression of ETRB2 (compare F to E: in F, the positive cells in blue occupy most of the microscopic field) and down-regulation of ETRB (G and H). (Bar = 90 μm.)