Table 1.
Clonal efficiency and phenotypic analysis of NC precursors
| Precursor type | Clonal efficiency, % of clones from total plated cells
|
||
|---|---|---|---|
| Control | ET3 | ET3/control | |
| Mel | 0.57 | 9.1 | ×16 |
| Glia-Mel | 1.4 | 18.8 | ×13.4 |
| Glia | 9.1 | 19.4 | ×2.1 |
| Glia-Neuro | 5.4 | 5.7 | n.s. |
| Glia-Mel-Neuro | 1.4 | 0.5 | n.s. |
| No marker | 6.5 | 7.4 | n.s. |
| Total | 24.6 | 61.1 | ×2.5 |
The clonal efficiency of NCC, defined by the percentage of total plated cells that give rise to a colony, was calculated in d9-clonal cultures grown in the absence (control) and in the presence of 100 nM ET3 (ET3). Precursor types were categorized according to cell phenotypes in their progeny (see Materials and Methods). Values were obtained from four different experiments containing 350 control and 175 treated cultures. Differences between treated and control values are expressed as the ratio of ET3 over control values (ET3/control). Only those that were statistically significant (P < 0.01) are indicated here. n.s., not significant.