Table 2.
Frequency of large colonies derived from NCC
| Precursor type | Frequency of large colonies (% of large clones from total clones)
|
||
|---|---|---|---|
| Control | ET3 | Δ% | |
| Glia | 1/32 (3.1) | 19/34 (55.9) | +52.8* |
| Glia-Mel + Mel | 0/7 (0) | 35/49 (71.4) | +71.4* |
| Glia-Neuro + | |||
| Glia-Mel-Neuro | 8/24 (33) | 8/11 (72.7) | +39.7 |
| No marker | 0/23 (0) | 3/13 (23) | +23* |
| Total | 9/86 (10.5) | 65/107 (60.7) | +50.2* |
A total of 193 clones (86 in control medium and 107 in the presence of ET3, same clones as in Table 1) was examined at d9 for the total cell number and cell phenotypes. Clones containing more than 1 × 103 cells were categorized as large clones. Values are expressed as the proportion and percent (%) of large clones from the total number of clones generated by each precursor type. Differences between ET3 and control values (Δ%) are indicated by ∗ when statistically significant (P < 0.05).